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MSH6 Antibody

SKU : BHA10558146

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA125723
Alternative Names	DNA mismatch repair protein Msh6; MutS-alpha 160 kDa subunit; G/T mismatch-binding protein; GTBP; GTMBP
Categories	Primary Antibodies
Clonality	polyclonal
Description	Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.Acharya S., Proc. Natl. Acad. Sci. U.S.A. 93:13629-13634(1996).Shiwaku H.O., DNA Res. 4:359-362(1997).Palombo F., Science 268:1912-1914(1995).
Host	Rabbit
Immunogen	Synthesized peptide derived from internal of Human MSH6.
Involvement In Disease	Hereditary non-polyposis colorectal cancer 5 (HNPCC5); Endometrial cancer (ENDMC); Mismatch repair cancer syndrome (MMRCS); Colorectal cancer (CRC)
Raised In	Rabbit
Reactivity	Human, Mouse
Regulatory	RUO
Relevance	Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction. Acharya S., Proc. Natl. Acad. Sci. U.S.A. 93:13629-13634(1996). Shiwaku H.O., DNA Res. 4:359-362(1997). Palombo F., Science 268:1912-1914(1995).
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of total MSH6 protein.
Subcellular Location	Nucleus, Chromosome

Uniprot	P52701
Database Links	HGNC:7329 OMIM:114500 KEGG:hsa:2956 STRING:9606.ENSP00000234420 UniGene:Hs.445052
Function	Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch
Pathway	DNA repair pathway
Protein Families	DNA mismatch repair MutS family

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:3000

Tested Application

ELISA,WB;WB:1:500-1:3000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10558146	CSB-PA125723	100ul	$297		Add to Cart

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