Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC). Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
Recombinant full-length human MSH2 protein was used as the immunogen for the MSH2 antibody.
Isotype
Rabbit IgG
Species Reactivity
Human
Note
Optimal dilution of the MSH2 antibody should be determined by the researcher.
Uniprot
IDP43246
Format
Purified
Purity
Protein A/G affinity
Storage
Aliquot the MSH2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Applications
WB, IHC-P
Description
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml