MutS homolog 2 is a protein that in humans is encoded by the MSH2 gene, which is located on chromosome 2. It is a tumor suppressor gene and more specifically a caretaker gene that codes for a DNA mismatch repair (MMR) protein, MSH2 forms a heterodimer with MSH6 to make the human MutS<font face=symbol>a</font> mismatch repair complex. It also dimerizes with MSH3 to form the MutS<font face=symbol>b</font> DNA repair complex. MSH2 is involved in many different forms of DNA repair, including transcription-coupled repair, homologous recombination, and base excision repair. It has been found that it may also be a coactivator of ESR1-dependent gene expression.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Human partial recombinant protein (AA 337-583) was used as the immunogen for this MSH2 antibody.
Isotype
IgG
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Subcellular Location
Nuclear
Gene Id
4436
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the MSH2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P, ICC, IF, FACS
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells