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Home / Primary Antibodies

MRPS27 Antibody

SKU : BHA10558491

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA022313
Alternative Names	mitochondrial 28S ribosomal protein S27; mitochondrial ribosomal protein S27; MRP-S27; RT27; S27mt
Categories	Primary Antibodies
Clonality	polyclonal
Description	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms.Nagase T., DNA Res. 3:321-329(1996).Koc E.C., Protein Sci. 10:471-481(2001).
Host	Rabbit
Immunogen	Synthesized peptide derived from internal of Human MRPS27.
Raised In	Rabbit
Reactivity	Human, Mouse
Regulatory	RUO
Relevance	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms. Nagase T., DNA Res. 3:321-329(1996). Koc E.C., Protein Sci. 10:471-481(2001).
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of total MRPS27 protein.
Subcellular Location	Cytoplasm, Mitochondrion

Uniprot	Q92552
Database Links	HGNC:14512 OMIM:611989 KEGG:hsa:23107 STRING:9606.ENSP00000261413 UniGene:Hs.482491
Function	RNA-binding component of the mitochondrial small ribosomal subunit (mt-SSU) that plays a role in mitochondrial protein synthesis
Protein Families	Mitochondrion-specific ribosomal protein mS27 family
Tissue Specificity	Overexpressed in hepatocellular carcinoma tissues compared with adjacent non-tumoral liver tissues (at protein level) (PubMed:28714366). Ubiquitous (PubMed:22841715).

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:3000

Tested Application

ELISA,WB;WB:1:500-1:3000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10558491	CSB-PA022313	100ul	$297		Add to Cart

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