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Home / Antibodies

MRPS25 Antibody

SKU : BHA10558490

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA019909
Alternative Names	mitochondrial 28S ribosomal protein S25; mitochondrial ribosomal protein S25; MRP-S25; RPMS25; RT25
Categories	Primary Antibodies
Clonality	polyclonal
Description	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4.Ota T., Nat. Genet. 36-40-45(2004).Hattori A., DNA Res. 7:357-366(2000).Koc E.C., J. Biol. Chem. 275:32585-32591(2000).
Host	Rabbit
Immunogen	Synthesized peptide derived from internal of Human MRPS25.
Raised In	Rabbit
Reactivity	Human, Mouse
Regulatory	RUO
Relevance	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Ota T., Nat. Genet. 36-40-45(2004). Hattori A., DNA Res. 7:357-366(2000). Koc E.C., J. Biol. Chem. 275:32585-32591(2000).
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of total MRPS25 protein.
Subcellular Location	Mitochondrion

Uniprot	P82663
Database Links	HGNC:14511 OMIM:611987 KEGG:hsa:64432 STRING:9606.ENSP00000253686 UniGene:Hs.657764
Protein Families	Mitochondrion-specific ribosomal protein mS25 family

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:3000

Tested Application

ELISA,WB;WB:1:500-1:3000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10558490	CSB-PA019909	100ul	$297		Add to Cart

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