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Home / Primary Antibodies

MRPL50 Antibody

SKU : BHA10558499

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA147326
Alternative Names	flj20493; flj21990; mitochondrial ribosomal protein l50; mrp-l50; mrpl50
Categories	Primary Antibodies
Clonality	polyclonal
Description	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q.Ota T., Nat. Genet. 36:40-45(2004).Humphray S.J., Nature 429:369-374(2004).The MGC Project Team; Genome Res. 14:2121-2127(2004).
Host	Rabbit
Immunogen	Synthesized peptide derived from internal of Human MRPL50.
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q. Ota T., Nat. Genet. 36:40-45(2004). Humphray S.J., Nature 429:369-374(2004). The MGC Project Team; Genome Res. 14:2121-2127(2004).
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of total MRPL50 protein.
Subcellular Location	Mitochondrion

Uniprot	Q8N5N7
Database Links	HGNC:16654 OMIM:611854 KEGG:hsa:54534 STRING:9606.ENSP00000363999 UniGene:Hs.288224
Protein Families	Mitochondrion-specific ribosomal protein mL50 family

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:3000

Tested Application

ELISA,WB;WB:1:500-1:3000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10558499	CSB-PA147326	100ul	$297		Add to Cart

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