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Home / Primary Antibodies

MRPL32 Antibody

SKU : BHA10559430

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA588561
Alternative Names	39S ribosomal protein L32; mitochondrial [Precursor]; L32mt; MRP-L32; HSPC283
Categories	Primary Antibodies
Clonality	polyclonal
Description	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.Suzuki T., J. Biol. Chem. 276:21724-21736(2001).The MGC Project Team; Genome Res. 14:2121-2127(2004).Kenmochi N., Genomics 77:65-70(2001).
Host	Rabbit
Immunogen	Synthesized peptide derived from internal of Human MRPL32.
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. Suzuki T., J. Biol. Chem. 276:21724-21736(2001). The MGC Project Team; Genome Res. 14:2121-2127(2004). Kenmochi N., Genomics 77:65-70(2001).
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of total MRPL32 protein.
Subcellular Location	Mitochondrion

Uniprot	Q9BYC8
Database Links	HGNC:14035 OMIM:611839 KEGG:hsa:64983 STRING:9606.ENSP00000223324 UniGene:Hs.50252
Protein Families	Bacterial ribosomal protein bL32 family

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IF

Description

ELISA ; WB ; IF, WB ; ELISA ; IF

Dilution

WB	1:500-1:3000
IF	1:100-1:500

Tested Application

ELISA,WB,IF;WB:1:500-1:3000,IF:1:100-1:500

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10559430	CSB-PA588561	100ul	$297		Add to Cart

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