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Home / Primary Antibodies

MLH1 Antibody / MutL Homolog 1 (V9166)

SKU : BHA17119556

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NSJ Bioreagents

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Mfr.No.	V9166, V9166-20UG, V9166-100UG
Clonality	Monoclonal (mouse origin)
Description	This MAb recognizes a protein of 83kDa, identified as MLH1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host	Mouse
Immunogen Region	Recombinant full-length human MLH1 protein was used as the immunogen for the MLH1 antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	Optimal dilution of the MLH1 antibody should be determined by the researcher.

Uniprot	P40692

Format	Purified
Purity	Protein A/G affinity
Storage	Aliquot the MLH1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Applications	IHC-P
Description	Immunohistochemistry (FFPE): 1-2ug/ml

Product Manual	Datasheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17119556-1	V9166-20UG	20ug	$219		Add to Cart
BHA17119556-2	V9166-100UG	100ug	$499		Add to Cart

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