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Home / Primary Antibodies

Mitochondrial Marker Antibody (V2352SAF)

SKU : BHA17123709

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NSJ Bioreagents

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Mfr.No.	V2352SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	Clone 113-1 antibody recognizes a 60kDa antigen that is a mitochondrial marker in human cells. Immunostaining produces a spaghetti-like patte in normal and malignant cells. This mitochondrial marker antibody is an excellent marker for human cells in xenographic model research. Abundant granular eosinophilic cytoplasm is a common feature of renal oncocytoma, chromophobe renal cell carcinoma, eosinophilic variant of papillary renal cell carcinoma, and the granular variant of clear cell renal cell carcinoma (RCC). The chief reason for their distinction from one another is the difference in their biologic behavior. However, precise characterization may be difficult in some cases because of overlapping morphologic features. Immunostaining patte with this mitochondrial marker antibody has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Salivary gland tumors usually show great variability both in their morphopathology as well as their clinical behavior. One study highlights the usefulness of this mitochondrial marker antibody to facilitate the classification of salivary tumors, an aspect that may sometimes have not only diagnostic implications, but also prognostic.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	A semi-purified mitochondrial preparation was used as the immunogen for this Mitochondrial marker antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	Differences in protocols and secondaries may require the Mitochondrial marker antibody 113-1 to be titered for optimal performance.1. FFPE staining is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Mitochondrial Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, IF, IHC-P
Description	Immunofluorescence: 1-2ug/ml,Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17123709	V2352SAF-100UG	100 ug	$499		Add to Cart

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