Mouse Anti-MethylglyoXal (MG) Monoclonal IgG2a

Mfr.No.	SMC-516, SMC-516D, SMC-516D-A390, SMC-516D-A488, SMC-516D-A594, SMC-516D-ALP, SMC-516D-APC, SMC-516D-BI, SMC-516D-FITC, SMC-516D-HRP, SMC-516D-PCP, SMC-516D-RPE, SMC-516S
Alternative Names	Methylglyoxal Antibody, 2-Oxopropanal Antibody, 2 oxo propanal Antibody, MG Antibody, Pyruvaldehyde Antibody, Methylglyoxal (MG) Antibody, MG-modified protein Antibody
Antigen	Methylglyoxal
Categories	Antibodies
Certificate Of Analysis	A 1:1000 dilution of SMC-516 was sufficient for detection of Methylglyoxal in 0.5 ug of Methylglyoxal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
Clonality	Monoclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Alkaline Phosphatase, APC, Biotin, FITC, HRP, PerCP, RPE
Description	Mouse Anti-Methylglyoxal (MG) Monoclonal IgG2a
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).
Immunogen Region	Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).
Isotype	IgG2a
Launch Date	2017/03/23
Net Weight (g)	0.1
Reactivity	Species Independent
Recombinant	FALSE
Regulatory	RUO
Species Reactivity Medium	Species Independent

Target	Methylglyoxal
Clone No	9E7
Research Area	Cancer, Oxidative Stress, Lipid peroxidation

Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); ICC/IF (1:50); FACS (1:50); FCM (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Methylglyoxal is an alpha-oxoaldehyde generated from lipid peroxidation that reacts with proteins to form adducts(1). It reacts with free amino acids and protein residues to generate advanced glycation endproducts (AGEs) and crosslinks DNA polymerase and substrate DNA to severely inhibit DNA replication (2).
Shipping Temperature	Blue Ice or 4°C
Specificity	Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C
Trademark	MOLECULAR SIGNATURE

Applications	ELISA, FACS, IHC, WB, ICC/IF, FCM
Description	WB ; ICC ; IF ; FACS ; FCM ; ELISA
Dilution	WB (1:1000); ICC/IF (1:50); FACS (1:50); FCM (1:50); ELISA (1:1000); IHC (1:50); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Negre-Salvayre et al. (2008) Br J Pharmacol. 153(1):6-20. 2. Murata-Kamiya, N., Kamiya, H. (2001) Nucleic Acids Res. 29(16): 3433-3438.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11905597-1	SMC-516D	Unconjugated	100 ug	$409	Add to Cart
BHA11905597-2	SMC-516D-A390	ATTO 390	100 ug	$463	Add to Cart
BHA11905597-3	SMC-516D-A488	ATTO 488	100 ug	$462	Add to Cart
BHA11905597-4	SMC-516D-A594	ATTO 594	100 ug	$462	Add to Cart
BHA11905597-5	SMC-516D-ALP	Alkaline Phosphatase	100 ug	$455	Add to Cart
BHA11905597-6	SMC-516D-APC	APC	100 ug	$461	Add to Cart
BHA11905597-7	SMC-516D-BI	Biotin	100 ug	$458	Add to Cart
BHA11905597-8	SMC-516D-FITC	FITC	100 ug	$453	Add to Cart
BHA11905597-9	SMC-516D-HRP	HRP	100 ug	$449	Add to Cart
BHA11905597-10	SMC-516D-PCP	PerCP	100 ug	$461	Add to Cart
BHA11905597-11	SMC-516D-RPE	RPE	100 ug	$459	Add to Cart
BHA11905597-12	SMC-516S	Unconjugated	12 ug	$44	Add to Cart

Methylglyoxal Antibody, Clone 9E7 - SMC-516