Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. (UniProt)
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Amino acids 195-319 were used as the immunogen for the MCM7 antibody.
Isotype
Mouse IgG2b, kappa
Species Reactivity
Human
Note
The stated application concentrations are suggested starting amounts. Titration of the MCM7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Uniprot
P33993
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the MCM7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, IHC-P
Description
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT