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Home / Antibodies

MART-1 / Melan-A Antibody Cocktail (V2118SAF)

SKU : BHA17123460

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NSJ Bioreagents

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Mfr.No.	V2118SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	This antibody cocktail recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody cocktail labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody cocktail does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Recombinant human protein (A103; M2-7C10; M2-9E3) was used as the immunogen for this MART-1 antibody cocktail.
Isotype	Mouse IgG1 (A103)+IgG2b (M2-7C10 & M2-9E3)
Species Reactivity	Human, Mouse, Rat
Note	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the MART-1 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, FACS, IF, IHC-P
Description	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17123460	V2118SAF-100UG	100 ug	$499		Add to Cart

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