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Home / Primary Antibodies

Map2k5 Antibody

SKU : BHA10557539

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA283905
Alternative Names	MAP kinase kinase 5; Map2k5; MAPKK 5; MAPK/ERK kinase 5;
Categories	Primary Antibodies
Clonality	polyclonal
Description	A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phospho-rylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resem-bles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphory-late ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7)phosphorylates and activates the JNK signal transduction pathway. Han, J., et al. 1996. J. Biol. Chem. 271: 2886-2891.Jiang, Y., et al. 1996. J. Biol. Chem. 271: 17920-17926.Tournier, C., et al. 1997. Proc. Natl. Acad. Sci. USA94: 7337-7442. Holland, P.M., et al. 1997. J. Biol. Chem. 272: 24994-24998.
Host	Rabbit
Immunogen	Peptide sequence around aa. 311-315(K-T-Y-V-G ) derived from Rat MEK5.
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phospho-rylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resem-bles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphory-late ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7)phosphorylates and activates the JNK signal transduction pathway. Han, J., et al. 1996. J. Biol. Chem. 271: 2886-2891. Jiang, Y., et al. 1996. J. Biol. Chem. 271: 17920-17926. Tournier, C., et al. 1997. Proc. Natl. Acad. Sci. USA94: 7337-7442. Holland, P.M., et al. 1997. J. Biol. Chem. 272: 24994-24998.
Species	Rattus Norvegicus (Rat)
Specificity	The antibody detects endogenous level of total MEK5 protein.
Subcellular Location	Isoform Beta: Cytoplasm, cytosol, SUBCELLULAR LOCATION: Isoform Alpha-1: Membrane, Note=The alternatively spliced exon in alpha isoform resemble conserved sequences that mediate interactions with the cytoskeleton, thereby explaining the differential localization, SUBCELLULAR LOCATION: Isoform Alpha-2: Membrane

Uniprot	Q62862
Database Links	KEGG:rno:29568 STRING:10116.ENSRNOP00000050528 UniGene:Rn.11054
Function	Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis (By similarity).
Pathway	MAPK signaling pathway Gap junction Neurotrophin signaling pathway
Protein Families	Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
Tissue Specificity	MEK5 beta is ubiquitously distributed with highest levels in the liver, whereas MEK5 alpha is expressed only in liver and brain.

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide
Purity	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:1000

Tested Application

ELISA,WB;WB:1:500-1:1000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10557539	CSB-PA283905	100ul	$297		Add to Cart

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