Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Binds p75NTR and antagonizes its association with TrkA, inhibits cell cycle progression, and facilitates p75NTR-mediated apoptosis. May act as a regulator of the function of DLX family members. May regulate p53 transcriptional activity and inhibit cell proliferation. Enhances p53 phosphorylation and accumulation.Zhao Z,et al.(2002) Sci China C Life Sci.45(4):412-20.Shen WG,et al.(2007) Cell Commun Adhes.14(1):21-31.Cheresh DA,et al. (1984) J Biol Chem.259(12):7453-9.
Host
Rabbit
Immunogen
Peptide with sequence C-EAEARAEARNRMGIGDE derived from Mouse Melanoma-associated antigen D1.
Raised In
Rabbit
Reactivity
Mouse, Rat
Regulatory
RUO
Relevance
Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Binds p75NTR and antagonizes its association with TrkA, inhibits cell cycle progression, and facilitates p75NTR-mediated apoptosis. May act as a regulator of the function of DLX family members. May regulate p53 transcriptional activity and inhibit cell proliferation. Enhances p53 phosphorylation and accumulation.
Zhao Z,et al.(2002) Sci China C Life Sci.45(4):412-20. Shen WG,et al.(2007) Cell Commun Adhes.14(1):21-31. Cheresh DA,et al. (1984) J Biol Chem.259(12):7453-9.
Species
Mus Musculus (Mouse)
Specificity
The antibody detects endogenous level of total Melanoma-associated antigen D1 protein.
Subcellular Location
Nucleus, Cytoplasm, Cell membrane, Peripheral membrane protein
Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3
Pathway
Neurotrophin signaling pathway
Tissue Specificity
Ubiquitously expressed in many adult tissues, except for the spleen. Expressed in osteoblastic and chondrogenic cell lines and also during embryonic development.
Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.