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Home / Primary Antibodies

LIN28B Antibody

SKU : BHA10557594

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA637784
Alternative Names	CSDD2; FLJ16517;
Categories	Primary Antibodies
Clonality	polyclonal
Description	Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma."Guo Y., Chen Y., Ito H., et al.Gene 384:51-61(2006) "Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA."Heo I., Joo C., Cho J., Ha M., Han J., Kim V.N. Mol. Cell 32:276-284(2008) "TUT4 in concert with Lin28 suppresses MicroRNA biogenesis through pre-microRNA uridylation."Heo I., Joo C., Kim Y.-K., Kim V.N.,et al. Cell 138:696-708(2009) "
Host	Rabbit
Immunogen	Peptide sequence around aa.242~246( P-S-V-Q-K) derived from Human LIN28B
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma." Guo Y., Chen Y., Ito H., et al.Gene 384:51-61(2006) "Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA." Heo I., Joo C., Cho J., Ha M., Han J., Kim V.N. Mol. Cell 32:276-284(2008) "TUT4 in concert with Lin28 suppresses MicroRNA biogenesis through pre-microRNA uridylation." Heo I., Joo C., Kim Y.-K., Kim V.N.,et al. Cell 138:696-708(2009) "
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of total LIN28B protein.
Subcellular Location	Nucleus, Nucleus, nucleolus, Cytoplasm

Uniprot	Q6ZN17
Database Links	HGNC:32207 OMIM:611044 KEGG:hsa:389421 STRING:9606.ENSP00000344401 UniGene:Hs.23616
Function	Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA
Protein Families	Lin-28 family
Tissue Specificity	Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver (PubMed:16971064). Isoform 1 is only detected in placenta and in moderately and poorly differentiated hepatocellular carcinoma cells (at protein level). Isoform 2 is

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:1000

Tested Application

ELISA,WB;WB:1:500-1:1000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10557594	CSB-PA637784	100ul	$297		Add to Cart

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