LCAT (Lecithin: Cholesterol Acyltransferase), is an enzyme that converts free cholesterol into cholesteryl ester. Azoulay et al.(1987) used a cDNA clone corresponding to LCAT to assign the locus to 16q22 through the analysis of DNA from somatic cell hybrids and in situ hybridization. LCAT plays an important role in lipoprotein metabolism, especially in the process termed reverse cholesterol transport The enzyme is synthesized in the liver and circulates in blood plasma as a complex with components of high density lipoprotein(HDL). Cholesterol from peripheral cells is transferred to HDL particles, esterified through the action of LCAT on HDL, and incorporated into the core of the lipoprotein. The cholesterol ester is thereby transported to the liver(Jonas, 2000).
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Amino acids 300-313 (RDFQRFFADLHFEE-human) were used as the immunogen for this LCAT antibody.
Isotype
IgG
Predicted Reactivity
Human, Rat
Reactivity
Human, Rat
Recombinant
No
Uniprot
P04180
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the LCAT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.