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Home / Primary Antibodies

Laminin gamma 1 Antibody / LAMC1 (V2683SAF)

SKU : BHA17124178

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NSJ Bioreagents

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Mfr.No.	V2683SAF-100UG
Clonality	Monoclonal (rat origin)
Description	Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This mAb reacts with laminin B2/1 chain of ~210kDa and does not cross-react with other basement membrane components or fibronectin. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This mAb stains uniformly all human and murine basement membranes.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Rat
Immunogen Region	A murine EHS laminin preparation was used as the immunogen for the Laminin gamma 1 antibody.
Isotype	Rat IgG2a, kappa
Species Reactivity	Human, Mouse
Note	Optimal dilution of the Laminin gamma 1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P11047

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Laminin gamma 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124178	V2683SAF-100UG	100 ug	$499		Add to Cart

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