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Home / Primary Antibodies

KRT8 Antibody / Cytokeratin 8 (V2664IHC)

SKU : BHA17124145

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NSJ Bioreagents

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Mfr.No.	V2664IHC-7ML
Clonality	Monoclonal (mouse origin)
Description	Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Anti-CK8 is useful for the differentiation of lobular (ring-like, perinuclear) from ductal (peripheral-predominant) carcinoma of the breast.
Formulation	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only
Host	Mouse
Immunogen Region	Recombinant human KRT8 protein was used as the immunogen for the Keratin 8 antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	Optimal dilution of the Keratin 8 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P05787

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Keratin 8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124145	V2664IHC-7ML	7 ml	$499		Add to Cart

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