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Home / Antibodies

KRT19 Antibody Cocktail / Cytokeratin 19 (V3132SAF)

SKU : BHA17124788

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NSJ Bioreagents

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Mfr.No.	V3132SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	This mAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	MCF-7 cells (A53-B/A2) and human mammary epithelial organoids (BA17) were used as the immunogen for this Cytokeratin 19 antibody cocktail.
Isotype	Mouse IgG1, kappa+IgG2a, lambda
Species Reactivity	Human
Note	The optimal dilution of the Cytokeratin 19 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P08727

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Cytokeratin 19 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, IHC-P
Description	Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124788	V3132SAF-100UG	100 ug	$499		Add to Cart

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