This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Human Cytokeratin 10 was used as the immunogen for this Cytokeratin 10 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human, Mouse
Note
Optimal dilution of the antibody should be determined by the researcher.
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the Cytokeratin 10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).