This mAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (KRT10). KRT10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of KRT10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, KRT10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), KRT10 is detected very frequently. Expression is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Recombinant human Cytokeratin 10 protein was used as the immunogen for the recombinant KRT10 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the recombinant KRT10 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
P13645
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the recombinant KRT10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IHC-P
Description
Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT