This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Formulation
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host
Mouse
Immunogen Region
Recombinant human Kappa light chain was used as the immunogen for this Kappa antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the Kappa Light Chain antibody should be determined by the researcher.
Format
CF488 Conjugate
Purity
Protein G affinity chromatography
Storage
Store the Kappa Light Chain antibody at 2-8oC, protected from light.
Applications
FACS
Description
Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood