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Home / Antibodies

Kappa Light Chain Antibody Cocktail (V3149IHC)

SKU : BHA17124818

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NSJ Bioreagents

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Mfr.No.	V3149IHC-7ML
Clonality	Monoclonal (mouse origin)
Description	This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Formulation	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only
Host	Mouse
Immunogen Region	Purified human Ig kappa chain (HP6053) and human B-lymphoma cells (L1C1) were used as the immunogens for this Kappa Light Chain antibody cocktail.
Isotype	Mouse IgG1, kappa+IgG1 kappa
Species Reactivity	Human
Note	The optimal dilution of the Kappa Light Chain antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P01601, P01834

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Kappa Light Chain antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124818	V3149IHC-7ML	7 ml	$499		Add to Cart

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