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Home / Antibodies

Involucrin Antibody (V2164)

SKU : BHA17111414

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Mfr.No.	V2164, V2164-20UG, V2164-100UG
Antigen	Involucrin
Clonality	Monoclonal
Description	This antibody recognizes a protein of 66-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this antibody reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between amino acids 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Mouse
Immunogen Region	Purified Involucrin from human keratinocytes was used as the immunogen for this antibody.
Isotype	IgG1,kappa
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No
Subcellular Location	Cytoplasmic

Gene Id	3713
Clone No	SY5

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein G affinity chromatography
Storage	Store the Involucrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	IHC-P
Dilution	Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT

Product Manual	Data Sheet

No Description Available.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17111414-1	V2164-20UG	20ug	$219		Add to Cart
BHA17111414-2	V2164-100UG	100ug	$499		Add to Cart

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