This antibody recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E-beta-7 integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. This antibody is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL).
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A portion of amino acids 775-855 from the human protein was used as the immunogen for the CD103 antibody.
Isotype
Mouse IgG2b, kappa
Species Reactivity
Human
Note
Titering of the CD103 antibody may be required for optimal performance.
Uniprot
P38570
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the CD103 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IHC-P
Description
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT