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Home / Antibodies

Insulin Antibody (V7133SAF)

SKU : BHA17126036

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NSJ Bioreagents

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Mfr.No.	V7133SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. (RefSeq)
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Recombinant INS protein was used as the immunogen for the Insulin antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human, Mouse, Rat
Note	Optimal dilution of the Insulin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P01308

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Insulin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17126036	V7133SAF-100UG	100 ug	$499		Add to Cart

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