IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor, which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development. IL-4 is expressed primarily by activated Th2 cells and NK cells, and at lower levels by mast cells, and basophils. IL-4 signals through the IL-4R, and dendritic cell IL-12.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rat
Immunogen Region
Partially purified native mouse IL-4 protein was used as the immunogen for this IL4 antibody.
Isotype
Rat IgG1, kappa
Species Reactivity
Mouse
Note
Optimal dilution of the antibody should be determined by the researcher.
Uniprot
P07750
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the IL-4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
ELISA, FACS, IF
Description
ELISA (order BSA/azide-free format),,Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml