This antibody is specific to IGF1 (Insulin like Growth Factor 1) and shows minimal cross-reaction with IGF11, Proinsulin, MSF, and Insulin. IGF1 is a polypeptide growth factor with two isoforms that are produced by alteative splicing. Isoform 1 is also known as IGF1B while isoform 2 is known as IGF1A. IGF1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of the IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Purified human IGF1 protein was used as the immunogen for this antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human, Mouse, Rat
Note
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the IGF1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
Neutralization, FACS, IF
Description
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,In-vitro neutralization of biological activity of IGF-1 (order BSA/sodium azide-free format)