AIF1 (Allograft inflammatory factor 1), also called IBA1 (Ionized calcium-binding adapter molecule 1) is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1/IBA1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, it colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding the protein resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A portion of amino acids 1-146 from the human protein was used as the immunogen for the recombinant IBA1 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Titering of the recombinant IBA1 antibody may be required for optimal performance.
Uniprot
P55008
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the recombinant IBA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IHC-P
Description
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT