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Home / Primary Antibodies / Monoclonal Antibodies

HSP90 Antibody, Clone 4F3.E8 - SMC-149

BHA11900680
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Email
[email protected]
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Swissprot P07900
Accession Number NP_001017963.2
Gene Id 3320
Target HSP90
Clone No 4F3.E8
Research Area Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Tumor Biomarkers, Protein Trafficking
Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:2000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms alpha and beta, which share 85% sequence amino acid homology. The two isoforms of HSP90 are expressed in the cytosolic compartment (1). Despite the similarities, HSP90alpha exists predominantly as a homodimer while HSP90beta exists mainly as a monomer (2). From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (3-6). Furthermore, HSP90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively.
HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation.
In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (9).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~90kDa. This antibody detects both alpha and beta forms of HSP90 equally well.
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications ELISA, IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP ; ELISA
Dilution WB (1:2000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Nemoto T. et al. (1997) J.Biol Chem. 272: 26179-26187.

2. Minami, Y, et al. (1991), J.Biol Chem. 266: 10099-10103.

3. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577.

4. Pearl H, et al. (2001) Adv Protein Chem 59: 157-186.

5. Neckers L, et al. (2002) Trends Mol Med 8: S55-S61.

6. Pratt W, Toft D. (2003) Exp Biol Med 228: 111-133.

7. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360.

8. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434.

9. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328.

10. Nemoto, T. (1997) Biochem and Mol. Bio Intl. 42 (5): 881-889.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900680-1 SMC-149B Unconjugated 200 ug $345
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BHA11900680-2 SMC-149A Unconjugated 50 ug $161
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BHA11900680-3 SMC-149B-A390 ATTO 390 200 ug $448
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BHA11900680-4 SMC-149B-A488 ATTO 488 200 ug $444
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BHA11900680-5 SMC-149B-A594 ATTO 594 200 ug $444
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BHA11900680-6 SMC-149B-ALP Alkaline Phosphatase 200 ug $432
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BHA11900680-7 SMC-149B-APC APC 200 ug $443
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BHA11900680-8 SMC-149B-BI Biotin 200 ug $435
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BHA11900680-9 SMC-149B-FITC FITC 200 ug $427
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BHA11900680-10 SMC-149B-HRP HRP 200 ug $419
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BHA11900680-11 SMC-149B-PCP PerCP 200 ug $443
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BHA11900680-12 SMC-149B-RPE RPE 200 ug $437
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BHA11900680-13 SMC-149S Unconjugated 12 ug $44
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