HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, <a href=../search_result.php?search_txt=nfkb>NFKB</a>, and <a href=../search_result.php?search_txt=tbet>TBET</a> expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through <a href=../search_result.php?search_txt=tlr2>TLR2</a>signaling.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Human partial recombinant protein (AA 260-496) was used as the immunogen for this HSP60 antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Gene Id
3329
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the HSP60 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P, ICC, IF, FACS
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (Paraffin): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence: 2-3ug/ml,Flow cytometry: 1-3ug/million cells