HSP60 Antibody - BHA17105117

Mfr.No.	R31750
Clonality	Polyclonal
Description	HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, <a href=../search_result.php?search_txt=nfkb>NFKB</a>, and <a href=../search_result.php?search_txt=tbet>TBET</a> expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through <a href=../search_result.php?search_txt=tlr2>TLR2</a>signaling.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	Human partial recombinant protein (AA 260-496) was used as the immunogen for this HSP60 antibody.
Isotype	IgG
Predicted Reactivity	Human, Mouse, Rat
Reactivity	Human, Mouse, Rat
Recombinant	No

Gene Id	3329

Format	Antigen affinity purified
Purification	Antigen affinity
Storage	After reconstitution, the HSP60 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	WB, IHC-P, ICC, IF, FACS
Dilution	Weste blot: 0.5-1ug/ml,Immunohistochemistry (Paraffin): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence: 2-3ug/ml,Flow cytometry: 1-3ug/million cells