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Home / Primary Antibodies / Monoclonal Antibodies

HSP40, Hdj1 Antibody, Clone 3B9.E6 - SMC-145

BHA11900640
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+1 866.986.9598
Email
[email protected]
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Swissprot P25685
Accession Number NP_006136.1
Gene Id 3337
Target HSP40
Clone No 3B9.E6
Research Area Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Protein Trafficking
Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~40kDa. Does not cross-react with HDJ2 or YDJ1.
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications ELISA, IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP ; ELISA
Dilution WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36.

2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316.

3. Sohn S.Y., Kim S.B., Kim J., and Ahn B.Y. (2006) J Gen Virol. 87(7): 1883-91.

4. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878.

5. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931.

6. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890.

7. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095.

8. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900640-1 SMC-145D Unconjugated 100 ug $295
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BHA11900640-2 SMC-145C Unconjugated 25 ug $156
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BHA11900640-3 SMC-145D-A390 ATTO 390 100 ug $349
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BHA11900640-4 SMC-145D-A488 ATTO 488 100 ug $348
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BHA11900640-5 SMC-145D-A594 ATTO 594 100 ug $348
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BHA11900640-6 SMC-145D-ALP Alkaline Phosphatase 100 ug $341
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BHA11900640-7 SMC-145D-APC APC 100 ug $347
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BHA11900640-8 SMC-145D-BI Biotin 100 ug $343
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BHA11900640-9 SMC-145D-FITC FITC 100 ug $339
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BHA11900640-10 SMC-145D-HRP HRP 100 ug $334
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BHA11900640-11 SMC-145D-PCP PerCP 100 ug $347
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BHA11900640-12 SMC-145D-RPE RPE 100 ug $344
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BHA11900640-13 SMC-145S Unconjugated 12 ug $44
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