It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Partially purified HSP27 protein from the human breast cancer cell line MCF-7 was used as the immunogen for this HSP27 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the antibody should be determined by the researcher.
Uniprot
P04792
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
FACS, IF, WB, IHC-P
Description
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT