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Home / Primary Antibodies / Polyclonal Antibodies

HSP22 Antibody - SPC-181

BHA11907120
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Email
[email protected]
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Swissprot Q9UJY1
Accession Number NP_055180.1
Gene Id 26353
Target HSP22
Research Area Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Neurodegeneration, Neuroscience, Protein Trafficking
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), ICC/IF (1:100), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Scientific Background HSP22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to HSP27. Similar to most other sHSPs, HSP22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). HSP22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HSPB8 interacted preferentially with a triple aspartate form of HSP27 which mimics HSP27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type HSP27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of HSP22 in various processes including proliferation, apoptosis and macro autophagy (4). Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~22kDa. Does not cross-react with HSP27 or alpha-crystallin.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP
Dilution WB (1:1000), ICC/IF (1:100), IHC (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Kappe G., et al. (2001) Biochem Biophys Acta. 1520: 1-6.

2. Benndorf R., et al. (2001) J Biol Chem. 276: 26753-26761.

3. Sun X., et al. (2004) J Biol Chem. 279: 2394-2402.

4. Kim M.V., et al. (2004) Biochem Biophys Res Commun. 325: 649-652.

5. Wilhelmus M.M., et al. (2006) Acta Neuropathol. (Berl) 111: 139-149.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11907120-1 SPC-181D Unconjugated 100 ul $276
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BHA11907120-2 SPC-181C Unconjugated 25 ul $125
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BHA11907120-3 SPC-181D-A390 ATTO 390 100 ul $330
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BHA11907120-4 SPC-181D-A488 ATTO 488 100 ul $329
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BHA11907120-5 SPC-181D-A594 ATTO 594 100 ul $329
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BHA11907120-6 SPC-181D-ALP Alkaline Phosphatase 100 ul $322
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BHA11907120-7 SPC-181D-APC APC 100 ul $328
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BHA11907120-8 SPC-181D-BI Biotin 100 ul $325
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BHA11907120-9 SPC-181D-FITC FITC 100 ul $320
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BHA11907120-10 SPC-181D-HRP HRP 100 ul $316
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BHA11907120-11 SPC-181D-PCP PerCP 100 ul $328
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BHA11907120-12 SPC-181D-RPE RPE 100 ul $326
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BHA11907120-13 SPC-181S Unconjugated 12 ul $44
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