Rabbit Anti-Plant HSP101/ClpB CT (Plant) Polyclonal

Mfr.No.	SPC-305, SPC-305B
Immunogen Species	Plant
Alternative Names	heat shock protein 1010 Antibody, HOT1 Antibody, HSP101 Antibody, HSPS Antibody
Antigen	HSP101
Categories	Antibodies
Cellular Localization	Cytoplasm
Certificate Of Analysis	1 ul/ml of SPC-305 was sufficient for detection of HSP101 in 2 ug of Arabidopsis thaliana WT lysate stressed at 38°C for 1.5 hours by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Conjugate	Unconjugated
Description	Rabbit Anti-Plant HSP101/ClpB CT (Plant) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Recombinant protein Arabidopsis thaliana HSP101/ClpB C-terminal
Immunogen Region	Recombinant protein Arabidopsis thaliana HSP101/ClpB C-terminal
Launch Date	2009/01/01
Net Weight (g)	0.2
Reactivity	Arabidopsis thaliana, Monocot, Plant
Recombinant	FALSE
Regulatory	RUO
Species	Plant
Species Reactivity Medium	Plant, Arabidopsis

Swissprot	P42730
Accession Number	NP_565083.1
Gene Id	843771
Target	HSP101
Research Area	Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Protein Trafficking

Buffer	Lyophilized rabbit Antiserum. For reconstitution add 200 µl of sterile water.
Dilutions	WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Rabbit Antiserum
Scientific Background	HSPs/chaperones are known to be expressed in plants not only when they experience high temperatures stress, but also in response to a wide range of other environmental stresses such as water stress, salt stress, cold stress and oxidative stress (1, 2). HSP100 is one such major class that acts as a chaperone. HSP101/ClpB is a member of this HSP100 family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones. In spite of expression during heat stress, members of HSP100 protein family are also expressed during seed development. HSP101 protein is localized in a cytoplasm.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects the C-terminal domain of HSP101 ~101kDa.
Storage	-20ºC
Storage Buffer	Lyophilized rabbit Antiserum. For reconstitution add 200 ul of sterile water.
Storage Temperature	-20°C

Applications	WB
Description	WB
Dilution	WB (1:1000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Vierling E. (1991) Annu Rev. Plant Physiol. Plant Mol. Biol. 42: 579-620. 2. Waters W., et al. (2004) Trends Plant Sci. 9: 244-252.

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Combination SKU	Supplier No.	Conjugate	Size	Your price	Quantity
BHA11907716-1	SPC-305B	Unconjugated	200 ul	$345		Add to Cart

HSP101/ClpB CT (Plant) Antibody - SPC-305