Rabbit Anti-Human HMGB1 Polyclonal

Mfr.No.	SPC-1259, SPC-1259D, SPC-1259D-A390, SPC-1259D-A488, SPC-1259D-A594, SPC-1259D-BI, SPC-1259D-FITC
Immunogen Species	Human
Alternative Names	Amphoterin Antibody, Chromosomal protein nonhistone HMG1 Antibody, High mobility group 1 Antibody, High mobility group protein B1 Antibody, HMG-1 Antibody, HMG3 Antibody, HMGB1 Antibody, HMGB1_HUMAN Antibody, SBP 1 Antibody, Sulfoglucuronyl carbohydrate binding protein Antibody
Categories	Antibodies
Cellular Localization	Nucleus, Chromosome, Cytoplasm, Endosome
Certificate Of Analysis	A 1:1000 dilution of SPC-1259 was sufficient for detection of HMGB1 in 10 ug of Jurkat, HepG2, COLO, Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human HMGB1 Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthesized peptide derived from internal of Human HMGB1., Homo Sapiens (Human)
Immunogen Region	Synthesized peptide derived from internal of human HMGB1.
Isotype	IgG
Launch Date	2016/09/06
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Human
Tissue Specificity	Ubiquituous. Expressed in platelets

Swissprot	P09429
Accession Number	NP_002119.1
Gene Id	3146
Target	HMGB1
Research Area	Cell Signaling, Epigenetics and Nuclear Signaling, Transcription, Domain Families, Hmg box

Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Dilutions	WB (1:1000), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Purity	Ubiquituous. Expressed in platelets
Scientific Background	HMGB1, also known as high mobility group box 1 protein, and functions much like histones as they are important chromatin proteins. In the nucleus, HMGB1 interacts with nucleosomes, transcription factors and histones. The presence of HMGB1 in the nucleus depends on postranslational modifications. Hyperacetylation on lysine residues causes it to translocate into the cytosol. HMGB1 is also secreted by immune cells and acts as a cytokine mediator of inflammation. The mechanism of inflammation and damae is its binding to TLR2. HMGB1 also interacts with P53 and has been proposed as a target for cancer therapy.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects endogenous levels of total HMGB1 protein.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	ELISA, WB
Description	WB ; ELISA
Dilution	WB (1:1000), ELISA (1:20000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Bianchi M.E., Agresti A. (2005). Curr. Opin. Genet. Dev. 15(5): 496506. 2. Klune J.R., Dhupar R., Cardinal J., Billiar T.R., Tsung A. (2008). Mol. Med. 14(7-8): 47684. 3. Imamura T., Izumi H., Nagatani G., Ise T., Nomoto M., Iwamoto Y., Kohno K. (2001). J. Biol. Chem. 276(10): 753440. 4. Lotze M.T., DeMarco R.A. (2003). Curr Opin Investig Drugs. 4(12): 14059.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11916884-1	SPC-1259D	Unconjugated	100 ul	$286	Add to Cart
BHA11916884-2	SPC-1259D-A390	ATTO 390	100 ul	$340	Add to Cart
BHA11916884-3	SPC-1259D-A488	ATTO 488	100 ul	$339	Add to Cart
BHA11916884-4	SPC-1259D-A594	ATTO 594	100 ul	$339	Add to Cart
BHA11916884-5	SPC-1259D-BI	Biotin	100 ul	$334	Add to Cart
BHA11916884-6	SPC-1259D-FITC	FITC	100 ul	$330	Add to Cart

HMGB1 Antibody - SPC-1259