Recognizes a DQ antigen, which is a dimer of 60kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
The T4-positive CTL clone HG-38 was used as the immunogen for the HLA-DQ antibody.
Isotype
Mouse IgG2a, kappa
Species Reactivity
Human
Note
Optimal dilution of the HLA-DQ antibody should be determined by the researcher.1. No special pretreatment is required for IHC staining.
Uniprot
P01908, P01909, P01920
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the HLA-DQ antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
ELISA, FACS, IF
Description
ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml