This mAb reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some auto-immune diseases are associated with certain class II alleles.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Human tonsil cells were used as the immunogen for the HLA-DP/DQ/DR antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the HLA-DP/DQ/DR antibody should be determined by the researcher.
Uniprot
P04440, P01908, P01909, P01920, P01903
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the HLA-DP/DQ/DR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, ELISA, FACS, IF, IHC-P
Description
ELISA: 2-4ug/ml for coating (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/ million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT