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Home / Primary Antibodies

HLA-Aw32 + HLA-A25 Antibody (V2574SAF)

SKU : BHA17124012

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NSJ Bioreagents

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Mfr.No.	V2574SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	This mAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31; B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This mAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, and DRw10 were used as the immunogen for the HLA-A25 antibody (HLA-Aw32).
Isotype	Mouse IgG2a, kappa
Species Reactivity	Human
Note	Optimal dilution of the HLA-A25 antibody (HLA-Aw32) should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P30443

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the HLA-A25 antibody (HLA-Aw32) at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124012	V2574SAF-100UG	100 ug	$499		Add to Cart

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