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Home / Primary Antibodies

Histone H1 Antibody (V7837SAF)

SKU : BHA17126984

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NSJ Bioreagents

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Mfr.No.	V7837SAF-100UG
Clonality	Recombinant Rabbit Monoclonal
Description	Please note that this antibody is a recombinant rabbit version of original anti-histone H1 antibody (clone AE-4). Because the variable heavy (VH) and variable light (VL) domains are the same, this recombinant antibody has the same exact reactivity as the original AE-4 MAb. There are several advantages of producing a recombinant version of a monoclonal antibody. For example, a recombinant antibody is a pure preparation of active immunoglobulin with no contaminating non-functional intact Ig or free light/heavy chains. Secondly, antibody can always be produced, even if hybridoma line is lost. Moreover, it adds the flexibility of converting the antibody to any species, isotype or format. Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical tu sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. This antibody is extensively used as a pan-nuclear marker.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Rabbit
Immunogen Region	Nuclei of human leukemia biopsy cells were used as the immunogen for this recombinant Histone H1 antibody.
Isotype	Rabbit IgG
Species Reactivity	Human, Mouse, Rat
Note	Optimal dilution of the antibody should be determined by the researcher.

Uniprot	P07305

Format	Purified
Purity	Protein A affinity chromatography
Storage	Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P, WB, FACS, IF
Description	Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Weste blot: 1-2ug/ml

Product Manual	<a href="https://www.nsjbio.com/tds-pdf/recombinant-histone-h1-antibody-ae-4-v7837" target="_blank">Datasheet</a>

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SKU	Supplier No.	Size	Your price	Quantity
BHA17126984	V7837SAF-100UG	100 ug	$499		Add to Cart

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