Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. (UniProt)
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
Recombinant full-length human protein was used as the immunogen for the rabbit monoclonal recombinant Histone H1 antibody.
Isotype
Rabbit IgG, kappa
Species Reactivity
Human, Mouse, Rat
Note
Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
P07305
Format
Purified
Purity
Protein A affinity chromatography
Storage
Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, FACS, IF, IHC-P
Description
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Weste blot: 1-2ug/ml