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Home / Primary Antibodies

Histone H1 Antibody (V3735SAF)

SKU : BHA17125543

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NSJ Bioreagents

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Mfr.No.	V3735SAF-100UG
Clonality	Recombinant Mouse Monoclonal
Description	Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical tu sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Nuclei of human leukemia biopsy cells were used as the immunogen for the recombinant Histone H1 antibody.
Isotype	Mouse IgG2a, kappa
Species Reactivity	Human, Mouse, Rat
Note	Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.2. View the non-recombinant version of this Histone H1 antibody.

Uniprot	P07305

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, FACS, IF, IHC-P
Description	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Weste blot: 1-2ug/ml

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17125543	V3735SAF-100UG	100 ug	$499		Add to Cart

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