It recognizes both the free and protein-conjugated (either soluble or cell bound) form of hexa-histidine. This MAb is highly specific to hexa-histidine and shows no cross-reaction with other structurally related compounds. It has a very high affinity for hexa-histidine and is excellent for use in various techniques. His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein s biochemical properties. A variety of plasmids contain DNA that encodes an amino-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. As is the case with other protein tag systems, this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
Hexa-histidine tagged human protein was used as the immunogen for the recombinant Poly His antibody.
Isotype
Rabbit IgG
Species Reactivity
Species independent
Note
Optimal dilution of the recombinant Poly His antibody should be determined by the researcher.
Uniprot
Not Applicable
Format
Purified
Purity
Protein A/G affinity
Storage
Aliquot the recombinant Poly His antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Applications
WB, FACS, IF
Description
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml