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Home / Primary Antibodies

HIF1A Antibody / HIF-1 alpha (V8220)

SKU : BHA17117732

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NSJ Bioreagents

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Mfr.No.	V8220, V8220-20UG, V8220-100UG
Clonality	Monoclonal
Description	HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host	Mouse
Immunogen Region	A portion of amino acids 239-530 from the human protein was used as the immunogen for the HIF1 alpha antibody.
Isotype	IgG2a,kappa
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No
Subcellular Location	Nuclear and cytoplasmic

Uniprot	Q16665
Clone No	Ha111a

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein G affinity chromatography
Storage	Store the HIF1 alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	ELISA, WB, IHC-P
Dilution	ELISA (order Ab without BSA for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17117732-1	V8220-20UG	20ug	$219		Add to Cart
BHA17117732-2	V8220-100UG	100ug	$499		Add to Cart

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