Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This mAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A portion of amino acids 311-462 from the human protein was used as the immunogen for this HER2 antibody.
Isotype
Mouse IgG2a, kappa
Species Reactivity
Human
Note
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER2 antibody to be titered up or down for optimal performance.
Uniprot
P04626
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IHC-P, FACS, IF
Description
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml