mAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
The human hepatocellular carcinoma cell line SK-H1A9-2 was used as the immunogen for this Hepatocyte Specific Antigen antibody.
Isotype
Mouse IgG2b, kappa
Species Reactivity
Human
Note
The optimal dilution of the Hepatocyte Specific Antigen antibody for each application should be determined by the researcher.
Uniprot
Not Known
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the Hepatocyte Specific Antigen antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
ELISA
Description
ELISA (For coating, purchase antibody without BSA)