This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. (UniProt)
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Amino acids 73-187 were used as the immunogen for the Granzyme B antibody.
Isotype
Mouse IgG2b, kappa
Species Reactivity
Human
Note
Optimal dilution of the antibody should be determined by the researcher.
Uniprot
P10144
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the Granzyme B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, IHC-P
Description
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml