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Home / Primary Antibodies

Granzyme B Antibody (V2564SAF)

SKU : BHA17123997

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NSJ Bioreagents

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Mfr.No.	V2564SAF-100UG
Clonality	Polyclonal (rabbit origin)
Description	Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'non-self' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Rabbit
Immunogen Region	A recombinant fragment (amino acids 73-187) from the human protein was used as the immunogen for the Granzyme B antibody.
Isotype	Rabbit IgG
Species Reactivity	Human
Note	Optimal dilution of the Granzyme B antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min

Uniprot	P10144

Format	Purified
Purity	Protein A affinity chromatography
Storage	Store the Granzyme B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, FACS, IF, IHC-P
Description	Flow Cytometry: 0.5-1ug/million cells in 0.1ml,Immunofluorescence: 0.5-1ug/ml,Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17123997	V2564SAF-100UG	100 ug	$499		Add to Cart

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