This mAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining patte of the Golgi zone in normal and malignant cells. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisteae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisteae.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
SU-DHL-1 large cell lymphoma cells were used as the immunogen for the Golgi antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the Golgi antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
Not Known
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the Golgi antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, FACS, IF, ICC, IHC-P
Description
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Weste blot: 1-2ug/ml,Immunocytochemistry (Acetone or paraformaldehyde fixed): 1-2ug/ml for 30 min,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT