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Home / Antibodies

Golgi Antibody (V2338SAF)

SKU : BHA17123693

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NSJ Bioreagents

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Mfr.No.	V2338SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	Clone 371-4 antibody specifically detects the Golgi complex in human cells. It is part of panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. The antibody recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain cell or tissue preparations and can be used as a marker in subcellular fractions. The antibody produces a diffuse staining patte of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. The 371-4 antibody is an excellent marker for human cells in xenographic model research.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	SU-DHL-1 large cell lymphoma cells were used as the immunogen.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Golgi complex antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	Not Known

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Golgi Complex antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, FACS, IF, ICC, IHC-P
Description	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Weste blot: 1-2ug/ml,Immunocytochemistry (Acetone or paraformaldehyde fixed): 1-2ug/ml for 30 min,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17123693	V2338SAF-100UG	100 ug	$499		Add to Cart

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